Separation, determination and composition profile of lipids in biodiesel using hyphenation of gradient-HPTLC with fluorescence detection by intensity changes and mass spectrometry
Researcher:
Jarne Lardiés, Carmen
Congress:
250st Americal Chemical Society National Meeting and Exposition
Participation type:
Póster
Year:
2015
Location:
Boston (EEUU)
Publication:
Prepr. Pap.-Am. Chem. Soc., Div. Energy Fuels, 60(2), 581, 2015
We report here an on-line, hyphenated High-Performance Thin-Layer Chromatography (HPTLC)based procedure on a unique silica gel plate that provides separation and quantitative determination of monoglycerides, diglycerides, and fatty acids in biodiesel (BX, X≥5), as well as their composition profile in the sample. Sequential steps of hyphenation include: AMD gradient separation of lipid classes (using a 4-step gradient based on t-butyl methyl ether-dichloromethane-n-heptane); followed by a postchromatographic impregnation of the plate with primuline (200 ppm), and densitometric detection and quantification, using Fluorescence Detection by Intensity Changes (FDIC, λexc= 365 nm). Finally, the peaks are directly transferred from the impregnated plate to an ESI-MS spectrometer using a head elution-based TLC-MS interface for peak identification and obtention of fatty acid-related composition profiles of each lipid class.
We report here an on-line, hyphenated High-Performance Thin-Layer Chromatography (HPTLC)based procedure on a unique silica gel plate that provides separation and quantitative determination of monoglycerides, diglycerides, and fatty acids in biodiesel (BX, X≥5), as well as their composition profile in the sample. Sequential steps of hyphenation include: AMD gradient separation of lipid classes (using a 4-step gradient based on t-butyl methyl ether-dichloromethane-n-heptane); followed by a postchromatographic impregnation of the plate with primuline (200 ppm), and densitometric detection and quantification, using Fluorescence Detection by Intensity Changes (FDIC, λexc= 365 nm). Finally, the peaks are directly transferred from the impregnated plate to an ESI-MS spectrometer using a head elution-based TLC-MS interface for peak identification and obtention of fatty acid-related composition profiles of each lipid class.