Array of fluorophores using a silica gel-based, high-throughput combinatorial platform. Part I: instrumental device and measurement
Researcher:
Jarne Lardiés, Carmen
Congress:
XVIII reunión de la Sociedad Española de Química Analítica.
Participation type:
Póster
Year:
2013
Location:
Úbeda (España)
The environment surroundings of a fluorophore affect its fluorescence due to non-specific (polarity) interactions. Several mathematical models have been proposed to relate the Stokes´ shift with the dielectric constant (e), the index of refraction (n) and/or other thermodynamic parameters characterizing the polarity of a medium. However, very little attention has been devoted to the environment effect in the fluorescence quantum yield (i.e. fluorescence intensity), but this effect applies for a lot of compounds interacting with many fluorophores. This has been used as the basis of detection systems for liquid chromatography and chemical sensors.
As a new analytical application, an array of 49 fluorophores (prepared on a silica gel plate) is here developed, aiming to display their interactions with potential analytes in a high throughput format to get a specific pattern response (fingerprint). Cholesterol and sphingomyelin have been chosen as analytes. In this communication (Part I) the experimental device and the very first experimental results are presented.
The environment surroundings of a fluorophore affect its fluorescence due to non-specific (polarity) interactions. Several mathematical models have been proposed to relate the Stokes´ shift with the dielectric constant (e), the index of refraction (n) and/or other thermodynamic parameters characterizing the polarity of a medium. However, very little attention has been devoted to the environment effect in the fluorescence quantum yield (i.e. fluorescence intensity), but this effect applies for a lot of compounds interacting with many fluorophores. This has been used as the basis of detection systems for liquid chromatography and chemical sensors.
As a new analytical application, an array of 49 fluorophores (prepared on a silica gel plate) is here developed, aiming to display their interactions with potential analytes in a high throughput format to get a specific pattern response (fingerprint). Cholesterol and sphingomyelin have been chosen as analytes. In this communication (Part I) the experimental device and the very first experimental results are presented.