Characterization of ten trinucleotide microsatellite loci in the Critically Endangered Pyrenean yam Borderea chouardii (Dioscoreaceae).
Segarra-Moragues, J.G.; Palop-Esteban, M.; González-Candelas, F.; Catalán, P.. Characterization of ten trinucleotide microsatellite loci in the Critically Endangered Pyrenean yam Borderea chouardii (Dioscoreaceae).. Molecular Ecology Notes. 2003, Vol. 3(2), p. 265-2003.
The low levels of allozymic variability found in the Critically Endangered Borderea chouardii
prompted us to develop microsatellite markers to assess the genetic variability and population
structure for the adequate conservation management of this species. A (CTT)nenriched
partial genomic library was constructed. Ten polymorphic microsatellite loci were
isolated from it, rendering 51 alleles in 47 individuals analysed. The allelic pattern
observed for all of the loci with more than two alleles suggests that B. chouardii is tetraploid.
The low levels of allozymic variability found in the Critically Endangered Borderea chouardii
prompted us to develop microsatellite markers to assess the genetic variability and population
structure for the adequate conservation management of this species. A (CTT)nenriched
partial genomic library was constructed. Ten polymorphic microsatellite loci were
isolated from it, rendering 51 alleles in 47 individuals analysed. The allelic pattern
observed for all of the loci with more than two alleles suggests that B. chouardii is tetraploid.