Controlling the Number of Proteins with Dip-Pen Nanolithography

Elena Bellido, Rocío de Miguel, Daniel Ruiz-Molina, Anabel Lostao, and Daniel Maspoch. Controlling the Number of Proteins with Dip-Pen Nanolithography. Advanced Materials; II: 8,4; Cuartil Q1 (Materials Science, Multidisciplinary) lugar 8 de 214, y Q1 de Nanoscience & Nanotechnology, lugar 4 de 59. 2010, Vol. 22 (3), p. 352-2010.

Desired numbers of proteins can be positioned on surfaces by using dip-pen nanolithography (DPN). Ferritin nanoarrays are fabricated by direct-writing nanodroplets of a ferritin solution on TEM surfaces. After the contact-angle value of these droplets is determined, the concentration of the inking protein solution and the diameter of the dot-like feature can be modulated to position a single ferritin particle on a surface (see figure).