Isolation and characterization of microsatellite loci in Acer opalus (Aceraceae), a sexually-polymorphic tree, through an enriched genomic library.
Segarra-Moragues, J.G.; Gleiser, G.; González-Candelas, F.. Isolation and characterization of microsatellite loci in Acer opalus (Aceraceae), a sexually-polymorphic tree, through an enriched genomic library.. Conservation Genetics. 2008, Vol. 9(4), p. 1059-2008.
An enrichment protocol was used to isolate and
characterize microsatellite loci in Acer opalus, a Mediterranean
tree species. Highly polymorphic microsatellite loci
were required for paternity analyses in a population of this
species. Eight microsatellite loci were amplified and a total
of 87 alleles were detected in a sample of 142 individuals
from one population, allowing the identification of each
individual with a unique multilocus genotype. The paternity
exclusion probabilities varied from 0.261 for locus
Aop820 to 0.806 for locus Aop450, and the parent-pair
exclusion probabilities varied from 0.433 for Aop820 to
0.940 for Aop450. The cumulative probabilities of exclusion
for paternity and parentage of the eight loci were both
higher than 0.999, supporting the usefulness of these
microsatellite loci for future paternity and parentage analyses
in A. opalus. Cross-species transferability was also
assayed, supporting their potential use in other eight Acer
species.
An enrichment protocol was used to isolate and
characterize microsatellite loci in Acer opalus, a Mediterranean
tree species. Highly polymorphic microsatellite loci
were required for paternity analyses in a population of this
species. Eight microsatellite loci were amplified and a total
of 87 alleles were detected in a sample of 142 individuals
from one population, allowing the identification of each
individual with a unique multilocus genotype. The paternity
exclusion probabilities varied from 0.261 for locus
Aop820 to 0.806 for locus Aop450, and the parent-pair
exclusion probabilities varied from 0.433 for Aop820 to
0.940 for Aop450. The cumulative probabilities of exclusion
for paternity and parentage of the eight loci were both
higher than 0.999, supporting the usefulness of these
microsatellite loci for future paternity and parentage analyses
in A. opalus. Cross-species transferability was also
assayed, supporting their potential use in other eight Acer
species.