Isolation and characterization of microsatellite loci in the Cape fynbos heath Erica coccinea (Ericaceae)
J. G. Segarra-Moragues; Donat-Caerols, S.; Ojeda, F.. Isolation and characterization of microsatellite loci in the Cape fynbos heath Erica coccinea (Ericaceae). Conservation Genetics. 2009, Vol. 10(6), p. 1815-2009.
<p>Enriched genomic libraries were used to isolate and characterize dinucleotide microsatellite loci in Erica coccinea, a South African Cape fynbos heath species with distinct resprouter and seeder populations. Microsatellites were required to investigate the effect of the contrasting demographic pattern driven by these two post-fire responses in the population genetic structure of seeder and resprouter forms within this species. Eight microsatellite loci were characterised and amplified a total of 106 alleles in 2 samples each of 30 individuals from 1 resprouter and 1 seeder population. Mean allele numbers were 7.88 and 11.0 for the resprouter and seeder population, respectively. Both populations showed similar average observed and expected heterozygosity levels, HO(resprouter) = 0.683, HO(seeder) = 0.696; HE(resprouter) = 0.726, HE(seeder) = 0.756, and average positive inbreeding coefficients FIS(resprouter) = 0.058, FIS(seeder) = 0.080. This set of microsatellite loci will be used to conduct a population genetic survey of seeder and resprouter populations throughout the range of the species. Cross-species transferability was also assayed in four other South African and four European species of the genus Erica, supporting their potential use for population genetic analyses.</p>
<p>Enriched genomic libraries were used to isolate and characterize dinucleotide microsatellite loci in Erica coccinea, a South African Cape fynbos heath species with distinct resprouter and seeder populations. Microsatellites were required to investigate the effect of the contrasting demographic pattern driven by these two post-fire responses in the population genetic structure of seeder and resprouter forms within this species. Eight microsatellite loci were characterised and amplified a total of 106 alleles in 2 samples each of 30 individuals from 1 resprouter and 1 seeder population. Mean allele numbers were 7.88 and 11.0 for the resprouter and seeder population, respectively. Both populations showed similar average observed and expected heterozygosity levels, HO(resprouter) = 0.683, HO(seeder) = 0.696; HE(resprouter) = 0.726, HE(seeder) = 0.756, and average positive inbreeding coefficients FIS(resprouter) = 0.058, FIS(seeder) = 0.080. This set of microsatellite loci will be used to conduct a population genetic survey of seeder and resprouter populations throughout the range of the species. Cross-species transferability was also assayed in four other South African and four European species of the genus Erica, supporting their potential use for population genetic analyses.</p>